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function [ data ] = getTiledData( obj, varargin )
%GETTILEDDATA Retrieves image data when the input is not tiled
% This method retrieves the image data (or a subset of it) in the case of
% images that multiple tiles. The user can specify subset
% of the images by specifying the dimension and the interval of interest
% as a Name-Value pair. If no arguments are given, all the data is
% extracted.
% INPUT:
% obj: the CZIReader instance
% NAME-VALUE ARGUMENTS
% 'Cols': Specify which columns to extract
% 'Rows': Specify which rows to extract
% 'C': Specify which channels to extract
% 'Z': Specify which planes to extract
% 'T': Specify which timeseries to extract
% 'S': Specify which series/position to extract
% 'TileRows': Specify which row tiles to read.
% 'TileCols': Specify which col tiles to read.
% 'tileSeparate': specify whether to keep tiles separate or to merge them
% into a single plane (default = false)
% OUTPUT:
% data: image data, up to 6 dimension (in this order: XYCZTS). If only one
% channel is extracted (or the input is single channel), the singleton
% dimension relative to channel is squeezed.
% EXAMPLES:
% data = obj.getTiledData(); %extract all data
% data = obj.getTiledData('C', 1:2); %extract data only from the first
% 2 channels
% data = obj.getTiledData('Rows', 1:2:obj.pixPerTileCol, 'Cols', 1:2:obj.pixPerTileRow); %
% extract data subsampled by a factor 2 in rows and cols
% data = obj.getTiledData('TileRows', 1:6, 'TileCols, 2:4) %Reads first six rows of
% tiles, and column tiles from 2 to 4
%parse input
p = inputParser();
p.KeepUnmatched = true;
p.addParameter('Cols', 1:obj.pixPerTileCol, @(x) isvector(x) && all(x > 0) && max(x) <= obj.width);
p.addParameter('Rows', 1:obj.pixPerTileRow, @(x) isvector(x) && all(x > 0) && max(x) <= obj.height);
p.addParameter('C', 1:obj.channels, @(x) isvector(x) && all(x > 0) && max(x) <= obj.channels);
p.addParameter('Z', 1:obj.stacks, @(x) isvector(x) && all(x > 0) && max(x) <= obj.stacks);
p.addParameter('T', 1:obj.time, @(x) isvector(x) && all(x > 0) && max(x) <= obj.time);
p.addParameter('S', 1:obj.series, @(x) isvector(x) && all(x > 0) && max(x) <= obj.series);
p.addParameter('TileCols', 1:obj.numTilesCol, @(x) isvector(x) && all(x > 0) && max(x) <= obj.numTilesCol);
p.addParameter('TileRows', 1:obj.numTilesRow, @(x) isvector(x) && all(x > 0) && max(x) <= obj.numTilesRow);
p.addParameter('tileSeparate', false, @(x) isscalar(x) && islogical(x));
p.parse(varargin{:});
rows = p.Results.Rows;
cols = p.Results.Cols;
channels = p.Results.C;
stacks = p.Results.Z;
timeseries = p.Results.T;
series = p.Results.S;
tileCols = p.Results.TileCols;
tileRows = p.Results.TileRows;
tileSeparate = p.Results.tileSeparate;
if obj.wrongMetadata % deal with messy indices
% Probably cannot get tiled data, because:
% A) some information is contradictory
% B) in case of stitching the tile position is not on a grid, we would
% have to read from all the tiles anyhow
if obj.verbose
warning('CZIReader.getTiledData: Metadata information is contradictory It''s probably better to read all data and subset afterwards.');
end
end
if tileSeparate
data = zeros(length(rows), length(cols), length(channels), length(stacks), ...
length(timeseries), length(series), length(tileRows), length(tileCols), obj.datatype);
else
sizeRows = round(length(rows) * (1 + (length(tileRows) - 1) * (1 - obj.tileOverlap)));
sizeCols = round(length(cols) * (1 + (length(tileCols) - 1) * (1 - obj.tileOverlap)));
data = zeros(sizeRows, sizeCols, length(channels), length(stacks), ...
length(timeseries), length(series), obj.datatype);
end
% get numelements in each dimension
nZ = numel(stacks);
nC = numel(channels);
nT = numel(timeseries);
nS = numel(series);
maxNum = nZ * nC * nT * nS;
% define progress bar
progBar = TextProgressBar('CZIReader --> Extracting data: ', 30);
%get index of start of each new tile
pixelStartTileRow = 1 + round((0:length(tileRows)-1) * (1 - obj.tileOverlap) * length(rows));
pixelStartTileCol = 1 + round((0:length(tileCols)-1) * (1 - obj.tileOverlap) * length(cols));
initialTileRow = tileRows(1);
initialTileCol = tileCols(1);
idxZ = 1;
for z = stacks
idxCh = 1;
for ch = channels
idxT = 1;
for t = timeseries
idxS = 1;
for s = series
% update progress bar
currNum = idxS + (idxT-1)*nS + (idxCh-1)*nS*nT + ...
(idxZ-1)*nS*nC*nT;
progBar.update(currNum/maxNum * 100);
%get directory entry
dirEntries = obj.directoryEntries(obj.dirEntryIndices{ch, z, t, s});
for k = 1:length(dirEntries)
currTileRow = obj.rowIndex(dirEntries(k).YPos);
currTileCol = obj.colIndex(dirEntries(k).XPos);
outTileRow = currTileRow - initialTileRow + 1;
outTileCol = currTileCol - initialTileCol + 1;
if any(currTileRow == tileRows) && any(currTileCol == tileCols)
% this tile is required!
tmpImg = obj.readRawSubblockSegm('dirEntry', dirEntries(k));
[rr, cc] = size(tmpImg(rows, cols));
if tileSeparate
data(:, :, idxCh, idxZ, idxT, idxS, currTileRow, currTileCol) = tmpImg(rows, cols);
else
data(pixelStartTileRow(outTileRow) : pixelStartTileRow(outTileRow) + rr - 1, ...
pixelStartTileCol(outTileCol) : pixelStartTileCol(outTileCol) + cc - 1, ...
idxCh, idxZ, idxT, idxS) = tmpImg(rows, cols);
end
end
end
idxS = idxS + 1;
end
idxT = idxT + 1;
end
idxCh = idxCh + 1;
end
idxZ = idxZ + 1;
end
%squeeze data, to remove singleton dimensions
data = squeeze(data);
end