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OpenFieldAnalysis/figure_histogram.m
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%FIGURE_HISTOGRAM generate a simple barplot, showing the fraction of time | |
% spent in the inner vs outer areas of the arena WT vs MT. | |
% | |
% Friedrich Kretschmer | |
% Max-Planck Institute for Brain Research | |
% sciclist@brain.mpg.de | |
clear variables | |
arenaSize = 1000; %square size of arena in mm | |
%Define various zones | |
innerZone1 = [300, 300, 400, 400]; | |
innerZone2 = [100, 100, 800, 800]; | |
innerZone3 = [50, 50, 900, 900]; | |
%Select zone to analyse in histogram | |
innerZone = innerZone1; %Select inner zone | |
fdir_wt = '/storage/scic/Data/External/People/Bea/set3/wt'; | |
fdir_mt = '/storage/scic/Data/External/People/Bea/set3/gad'; | |
fdir_result = '/storage/scic/Data/External/People/Bea/set3'; | |
%Collect from all wildtype files | |
d = dir([fdir_wt, filesep, '*.mat']); | |
tInner_wt = nan(size(d,1), 1); | |
tOuter_wt = nan(size(d,1), 1); | |
animalNumber_wt = nan(size(d,1), 1); | |
for i = 1:size(d,1) | |
fileName = [fdir_wt, filesep, d(i).name]; | |
s = whos('-file', fileName); | |
animalNumber_wt(i) = str2double(d(i).name(1:2)); | |
if ismember('mousePos', {s.name}) | |
load(fileName, 'mousePos', 'frameRate'); | |
frameOnset = round(60*frameRate); %Start at 1min | |
frameOffset = round(660*frameRate); %Stop at 11min | |
[nInnerZone, nOuterZone] = getInnerOuter(mousePos(frameOnset:frameOffset,:), innerZone); | |
tInner_wt(i) = nInnerZone/frameRate; | |
tOuter_wt(i) = nOuterZone/frameRate; | |
end | |
end | |
%Collect from all mutant files | |
d = dir([fdir_mt, filesep, '*.mat']); | |
tInner_mt = nan(size(d,1), 1); | |
tOuter_mt = nan(size(d,1), 1); | |
animalNumber_mt = nan(size(d,1), 1); | |
for i = 1:size(d,1) | |
fileName = [fdir_mt, filesep, d(i).name]; | |
s = whos('-file', fileName); | |
animalNumber_mt(i) = str2double(d(i).name(1:2)); | |
if ismember('mousePos', {s.name}) | |
load(fileName, 'mousePos', 'frameRate'); | |
frameOnset = round(60*frameRate); %Start at 1min | |
frameOffset = round(660*frameRate); %Stop at 11min | |
[nInnerZone, nOuterZone] = getInnerOuter(mousePos(frameOnset:frameOffset,:), innerZone); | |
tInner_mt(i) = nInnerZone/frameRate; | |
tOuter_mt(i) = nOuterZone/frameRate; | |
end | |
end | |
tInner = [tInner_wt; tInner_mt]; | |
tOuter = [tOuter_wt; tOuter_mt]; | |
animalNumber = [animalNumber_wt; animalNumber_mt]; | |
r_wt = tInner_wt./tOuter_wt; | |
r_mt = tInner_mt./tOuter_mt; | |
%Create plot | |
figure | |
bar(1:size(r_wt,1) , r_wt, 'k'); | |
hold on | |
bar(size(r_wt, 1)+1:size(r_wt, 1)+size(r_mt, 1), r_mt, 'r'); | |
legend({'Wildtype', 'Gad^{-/-}'}); | |
set(gca,'Xtick',1:numel(animalNumber)); | |
set(gca, 'XTickLabel', animalNumber); | |
ylabel ('Fraction of time spent in inner and outer area'); | |
xlabel('Animal'); | |
m = [animalNumber, tInner./tOuter]; | |
%Save result | |
csvwrite([fdir_result, filesep, 'zone_', mat2str(innerZone), '_ratios.csv'], m); | |
saveas(gcf, [fdir_result, filesep, 'zone_', mat2str(innerZone), '_histogram.pdf']); |