# # The action section # # clean - whether or not to skip the prinseq cleaning step (see below). # snv - whether or not to perform SNV analysis. # cnv - whether or not to perform CNV analysis. # container - whether or not to create a RDS container. # action: clean: True snv: True cnv: True container: True # # The samplesheet section # # file - The path to the SampleSheet text file. Set to None if no samplesheet is available # index - The name of the Column that should be used as index # case - The name of the Column containing the case identifier to which the index belongs to # samplesheet: file: 'SampleSheet.txt' index: 'Sample' case: 'Case' # # The data section # # Contains named dictionaries with input sequencing files: # r1 - The fastq file containing R1. # r2 - The fastq file containing R2. # data: sample1: r1: '' r2: '' # # The reference section # # URL - The URL from where the reference genome can be downloaded. Wildcards are allowed in curly brackets # organism - Wildcard for the reference organism # release - Wildcard for the reference release # file - Wildcard(s) for the reference files that should be merged into the final reference transcriptome # target - The path to a bed file with coordinates of the target regions # reference: annotation_URL: 'ftp.ebi.ac.uk/pub/databases/gencode/Gencode_{organism}/release_{release}/gencode.v{release}.{file}.fa.gz' genome_URL: 'ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_{organism}/release_{release}/{assembly}.primary_assembly.genome.fa.gz' organism: 'mouse' release: 'M17' assembly: 'GRCm38' file: - basic_annotation target: 'path/to/target_regions.bed' # # The directory section # # ref - directory name for reference files # fastq - directory name for fastq files # bam - directory name for STAR alignments # cnv - directory name for Copy Number Variation analysis # snv - directory name for Sequence Nucleotide Variation analysis # tables - directory name for aggregated tables # log - directory name for program logs # R - directory name for Rdata objects # dirs: ref: 'ref' fastq: 'fastq' bam: 'bam' cnv: 'cnv' snv: 'snv' tables: 'tables' log: 'log' R: 'rds' # # The debug section # debug: False