ATUMtomeUI

User interface to drive automated tape collecting ultramicrotome

Tape

motor

driver

tension motor

Parameters

Microtome (UC7)

• Cutting speed [mm/s] 0.1 – 2
• Cutting thickness (feed) [µm] 0.025 – 300
• Start/Stop cutting Motor off/on
• Counter window (nb cuts since start of series) -
• Total specimen advance (or advance left) [µm] 0-200 µm
• Return speed [mm/s] ?? (before: low, medium, high), I will try to figure out the real values
• Cutting window Start/end of cutting window
• N/S motor movement [µm] Relative movement (0.1 – 500)
• E/W motor movement [µm] Relative movement (0.1 – 500)

Camera

• Live-image
• gain
• exposure time
• ROI
• Snapshot
• Video recording

Water pump

• 1 button to activate pump
• Dispersed volume/activation: ?? (I will figure it out and send you the values)

Tape mechanism

• Tape speed: 0.1 – 2 mm/s (calibration required!!)
• Start/stop tape motors
• Tape tension: support motors on/off

Implementation

Classes

• MainWindow (controller)
• DriverVideoStream
• DriverMicrotome
• DriverWaterPump
• DriverTapeMechanism

Virtual class driver

• DriverAbstract
  • isRunning()
  • Start()
  • Stop()
  • Trigger()

Drawing a rectangle for ROI

Classes

• QGraphicsObject
• QPen
• QPainter
• QGraphicsScene
• QGraphicsSceneMouseEvent
• QRectF

Reference readings

Maxon motor EPOS2 tutorial

Maxon Motors

• BOTTOM = USB0
• TOP = USB1

Digital Outputs

• GREEN LIGHT = USB0, 0x0003, DIC_GENERAL_PURPOSE_C, PIN13
• SOUND = USB0, 0x0004, DIC_GENERAL_PURPOSE_D, PIN12
• SUPPORT MOTORS = USB1, 0x0003, DIC_GENERAL_PURPOSE_C, PIN13
• RED LIGHT = USB1, 0x0004, DIC_GENERAL_PURPOSE_D, PIN12