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FreezingAnalysis/Traditional/blobTrackBinarize.m
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% | |
% binarization | |
% | |
function [bw,u,uceroded] = blobTrackBinarize(g2,i,maskInside,q); | |
background = q.background; | |
useEnhancement = q.useEnhancement; | |
% i = 5600; | |
try | |
u= g2.read(i); | |
catch | |
disp(sprintf('Skipping frame %d for reading problems.',i)); | |
% sometimes the video reader marks frames tha do not actually exist! | |
bw = []; | |
u = []; | |
return | |
end | |
u = rgb2gray(u); | |
if ~isempty(background) | |
ub = double(u)-background; | |
else | |
ub = u; | |
end | |
ubReversed = imcomplement((ub)); | |
if useEnhancement | |
ucg = imadjust(ubReversed); | |
else | |
ucg = ubReversed; | |
end | |
% smarts | |
if ~isempty(maskInside) | |
% ucg = ucg./max(ucg(:)); | |
ucg = mbimg.mask.applyMask(ucg,maskInside); | |
end | |
useSingleErosion = true; | |
if useSingleErosion | |
% ucgi = (imerode(ucg,strel('diamond',20))); | |
% ucgi = ucgi./max(ucgi(:)); | |
% | |
% t = graythresh(ucgi); | |
% t = 0.8; | |
% | |
uceroded = imerode(ucg,strel('diamond',q.erosion)); | |
%bw = im2bw(mbimg.binarize(mbimg.normalize(uceroded),q.binarizationThreshold)); | |
bw = mbimg.binarize(mbimg.normalize(uceroded),q.binarizationThreshold); | |
return | |
end | |
if useEnhancement | |
% direct | |
ucgi = imerode(ucg,strel('diamond',5)); | |
t = graythresh(ucgi); | |
bw = im2bw(ucgi,t); | |
return | |
end | |
%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%%% | |
% | |
% elliminates ears | |
% | |
elliminateEars = true; | |
if elliminateEars | |
sec = strel('diamond',9); % structuring element | |
uh = imclose(ucg,sec); | |
else | |
uh = ucg; | |
end | |
% | |
% erode tails | |
% | |
see = strel('diamond',12); % structuring element | |
ue = imerode(uh,see); | |
ue = mat2gray(ue); | |
% gets blobs | |
%t = graythresh(ue); | |
%ue = mat2gray(ucg); | |
try | |
thresh = multithresh(ue,3); | |
catch | |
thresh = multithresh(ue,2); | |
end | |
t = thresh(end); | |
%ue = mbimg.mask.applyMask(ue,maskInside); | |
bwn = im2bw(ue,t); | |
% | |
% | |
% erode tails | |
% | |
% | |
seo = strel('diamond',12); % structuring element | |
bw = imopen(bwn,seo); | |
if length(find(bw))<30 | |
bw = bwn; | |
%error('Norl! the mouse disapeared!'); | |
end | |
if length(find(bw))<30 | |
%error('Norl! the mouse disapeared!'); | |
bw = []; | |
end | |