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Releases: jenzopr/scRNAseq.preprocessing

Initial version v0.1

10 Oct 13:12
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This release creates a referenceable version of the single-cell RNA-seq preprocessing pipeline.

Features include:

  • Automatic download of transcriptome reference(s) for mouse and human from Gencode.
  • Streamed cleaning of fastq files using htstream.
  • Expression quantification using salmon or kallisto (UMI-aware).
  • Automatic creation of a SingleCellExperiment container for downstream analysis in R, with statistics from preprocessing.

Upcoming

Upcoming releases will implement the following features:

  • Reformatting fastq header lines using the umis tool.
  • Demultiplexing of fastq files using pydemult.
  • S3 / Kubernetes support for cloud-based deployments.