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renewiegandt edited this page Mar 31, 2021 · 1 revision
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Scatter plot of bin comparison

scatter_annotation Plot 1 shows the gRNA pairs targeting exons. The color indicates the log2 fold change. The size of the dots indicates the number of pairs for the specific pair. Plot 2 shows the excisions found in the Nanopore reads. Plot 3 shows the combinations that are present in the gRNA pairs but are missing in the Nanopore reads. The color indicates the excision length. The size of the dots in plot 2 shows the number of pairs found for the specific pair. The pairs are separated into quadrants A, B, and C. These quadrants can be set as a parameter.

For each quadrant, static plots are generated.

exon_exon_counts_final_quadrants_present_points_percent
Percentage of found excisions compared to possible excisions per quadrant.
exon_exon_counts_final_boxplot_per_value
Boxplots of mean excision length and normalized NanoPore count per quadrant.

log2 fold change scatter

gRNA_scatter Each dot of the scatter plot indicates one gRNA pair, which is color-coded regarding its PAM orientation. The x-axis shows the excision length. The line plots show the density for the x- and y-axis.