Multi-omics single-nucleus analysis of the human orbitofrontal cortex in psychiatric disorders

We used a combinatorial approach merging clinical data with genetic susceptibility to provide new fundamental understanding of the biological basis of these disorders, specifically analyzing gene expression and chromatin accessibility at the single-cell level within the orbitofrontal cortex of 92 postmortem human brain samples.In our comprehensive study, we tackled these challenges by analyzing gene expression and chromatin accessibility at the single-cell level within the orbitofrontal cortex of 92 postmortem human brain samples. Our approach enriches the understanding of these disorders by complementing clinical data with genetic susceptibility. Through single-nucleus (sn) RNA-seq and snATAC-seq, we respectively examined approximately 800,000 and 400,000 nuclei. Our analysis revealed cell type-specific dysregulation related to clinical diagnosis and genetic risk across different cortical cell types.

This repository contains all scripts used for preprocessing (e.g. quality control, doublet removal, normalization, dimensionality reduction, clustering, cell type assignment) and differential analysis (gene expression and chromatin accessibility).