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comp-metadata/docs/quantification/chip-seq/CHPv0.xml
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<?xml version="1.0"?> | |
<?xml-stylesheet type="text/css" href="http://deep.mpi-inf.mpg.de/DAC/files/style/deep_process_style.css"?> | |
<process> | |
<name>CHP</name> | |
<version>0</version> | |
<author> | |
<name>Peter Ebert</name> | |
<email>pebert@mpi-inf.mpg.de</email> | |
</author> | |
<description> | |
ANDREAS: please insert general comments here | |
</description> | |
<inputs> | |
<filetype> | |
<identifier>ALNv0_histone.bam</identifier> | |
<format></format> | |
<quantity>collection</quantity> | |
<comment></comment> | |
</filetype> | |
<filetype> | |
<identifier>ALNv0_input.bam</identifier> | |
<format></format> | |
<quantity>single</quantity> | |
<comment></comment> | |
</filetype> | |
<filetype> | |
<identifier>ALNv0.bai</identifier> | |
<format></format> | |
<quantity>collection</quantity> | |
<comment>Index files are renamed internally to .bam.bai since deepTools is expecting index naming like this</comment> | |
</filetype> | |
</inputs> | |
<references> | |
<filetype> | |
<identifier>ref_genome</identifier> | |
<format>2bit</format> | |
<quantity>single</quantity> | |
<comment>The reference genome file</comment> | |
</filetype> | |
<filetype> | |
<identifier>chip_ctrl_regions</identifier> | |
<format>BED</format> | |
<quantity>single</quantity> | |
<comment>Control regions obtained from Freiburg for quality control of ChIPseq samples</comment> | |
</filetype> | |
</references> | |
<outputs> | |
<filetype> | |
<identifier>samplesID.PROCESS.DATE.corplot.png</identifier> | |
<format>deepTools graphics PNG</format> | |
<quantity>single</quantity> | |
<comment></comment> | |
</filetype> | |
<filetype> | |
<identifier>samplesID.PROCESS.DATE.fgprplot.png</identifier> | |
<format>deepTools graphics PNG</format> | |
<quantity>single</quantity> | |
<comment></comment> | |
</filetype> | |
<filetype> | |
<identifier>sampleID.PROCESS.DATE.gcbplot.png</identifier> | |
<format>deepTools graphics PNG</format> | |
<quantity>collection</quantity> | |
<comment></comment> | |
</filetype> | |
<filetype> | |
<identifier>sampleID.PROCESS.DATE.gcbfreq.txt</identifier> | |
<format></format> | |
<quantity>collection</quantity> | |
<comment></comment> | |
</filetype> | |
<filetype> | |
<identifier>sampleIDs.PROCESS.DATE.bamcomp.bw</identifier> | |
<format></format> | |
<quantity>collection</quantity> | |
<comment></comment> | |
</filetype> | |
</outputs> | |
<software> | |
<tool> | |
<name>bamCorrelate (deepTools)</name> | |
<version>1.5.1-252-gbbb0711</version> | |
<command_line><![CDATA[ bamCorrelate bins -p {numproc} --bamfiles {ALNv0_histone.bam} {ALNv0_input.bam} --labels {labels} --plotFile {samplesID.PROCESS.DATE.corplot.png} --fragmentLength {fragment_length} --corMethod {correlation_method} ]]></command_line> | |
<loop>no looping</loop> | |
<comment></comment> | |
</tool> | |
<tool> | |
<name>bamFingerprint (deepTools)</name> | |
<version>1.5.1-252-gbbb0711</version> | |
<command_line><![CDATA[ bamFingerprint {numproc} {bamfiles} --labels {labels} --plotFile {samplesID.PROCESS.DATE.fgprplot.png} --fragmentLength {fragment_length} ]]></command_line> | |
<loop>no looping</loop> | |
<comment></comment> | |
</tool> | |
<tool> | |
<name>computeGCBias (deepTools)</name> | |
<version>1.5.1-252-gbbb0711</version> | |
<command_line><![CDATA[ computeGCBias -p {numproc} --bamfile {ALNv0_*.bam} --effectiveGenomeSize {genomesize} --genome {reference_genome} --GCbiasFrequenciesFile {sampleID.PROCESS.DATE.gcbfreq.txt} --biasPlot {sampleID.PROCESS.DATE.gcbplot.png} ]]></command_line> | |
<loop>no looping</loop> | |
<comment></comment> | |
</tool> | |
<tool> | |
<name>MACS2</name> | |
<version>macs2 2.0.10.20130915 (tag:beta)</version> | |
<command_line><![CDATA[ macs2 callpeak -f BAM {bamfile} {control} --gsize {genomesize} {outname} --keep-dup all --nomodel --extsize 200 --qvalue 0.01 ]]></command_line> | |
<loop>no looping</loop> | |
<comment></comment> | |
</tool> | |
<tool> | |
<name>bamCompare (deepTools)</name> | |
<version>1.5.1-252-gbbb0711</version> | |
<command_line><![CDATA[ bamCompare {numproc} {bamfile1} {bamfile2} --outFileName sampleIDs.PROCESS.DATE.bamcomp.bw --outFileFormat bigwig --fragmentLength 200 --scaleFactorsMethod SES ]]></command_line> | |
<loop>no looping</loop> | |
<comment></comment> | |
</tool> | |
<tool> | |
<name>bamCoverage (deepTools)</name> | |
<version>1.5.1-252-gbbb0711</version> | |
<command_line><![CDATA[ bamCoverage {numproc} {bamfile} --outFileName sampleID.PROCESS.DATE.bamcov.bw --outFileFormat bigwig --fragmentLength 200 ]]></command_line> | |
<loop>no looping</loop> | |
<comment></comment> | |
</tool> | |
</software> | |
</process> |