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cluster PolyA supported sites from 3'End sequencing

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PASSFinder

Dependencies

Install HTSLib, so that include files are available under /usr/local/include and library file is available under /usr/local/lib. Make sure that those paths are in your search path.

Linux: from source and follow instructions

Mac: brew install htslib

Install Zlib

Linux: apg-get install zlib1g-dev

Mac:

either install developer tools with xcode-select --install

or use Homebrew brew install zlib

Compilation

Makefile is provided. make command will create an executable.

Help

Tool: PASSFinder

Version: v0.01

Summary: Cluster PolyA supported sites from 3'End sequencing.

Usage:

PASSFinder [OPTIONS] -i/--input <bam/stdin> -m/--maskpoly <bed> -o/--output <bed/stdout>

Options:

-i/--input BAM/stdin file [required]
-o/--output BED/stdout file [required]
-m/--maskpoly BED/stdin file with genomic poly regions [required]
-w/--window	sliding window size
-d/--depth	minimum number of polyA reads to define PASS
-p/--polysize	minimum number of As for tail and Ts for head to define poly stretch
-h/--help	print help message
-v/--version	print current version

Bug reports

Scientific Computing Facility @ Max-Planck Institute For Brain Research

sciclist@brain.mpg.de

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