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Estimation motifs #92

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Mar 26, 2019
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170ca1e
get_motif_seq.R: Removed redundant code
renewiegandt Jan 28, 2019
565d4b8
Rename get_motif_seq.R
renewiegandt Jan 28, 2019
e470bed
pipeline.nf: changed output dir of logs
renewiegandt Jan 28, 2019
b931ea7
compareBed.sh: rename stats to log
renewiegandt Jan 28, 2019
35d6f0c
Added venn.R to 3.2_evaluation
renewiegandt Feb 4, 2019
98654e1
Update uropa.config
renewiegandt Feb 4, 2019
2186cc6
Added uropa to the enviroment yaml
renewiegandt Feb 4, 2019
3793c70
Added uropa and venn process; mergeing gtf files; added parameter for…
renewiegandt Feb 4, 2019
1a95d4c
Added yaml-file for meme-suite enviroment
renewiegandt Feb 11, 2019
011b99a
Added meme_env parameter
renewiegandt Feb 11, 2019
10a3df3
Fixed channel for venn; added conda meme-env
renewiegandt Feb 11, 2019
4c08eeb
added parameter gap penalty to 2.2config
renewiegandt Feb 11, 2019
9bf3ed9
merge_similar_clusters.R: bugfix
renewiegandt Feb 11, 2019
50ec488
fixed label_cluster; minor changes
renewiegandt Feb 11, 2019
e11e0af
merge_similar_clusters: bugfix; improved cluster plot
renewiegandt Mar 4, 2019
a46476f
pipeline: added uropa summary to output
renewiegandt Mar 4, 2019
51dcfc0
merge_similar_clusters: fixed filenames
renewiegandt Mar 4, 2019
f1eb5f1
pipeline: Added seed for glam2
renewiegandt Mar 4, 2019
c034878
Added skript png_to_pdf
renewiegandt Mar 20, 2019
fae46c6
Better output directory names
renewiegandt Mar 20, 2019
cdc73a2
Minor formating changes of output file
renewiegandt Mar 20, 2019
89ae057
Update parameters in README
renewiegandt Mar 26, 2019
f8c572b
Added new parameters to config files
renewiegandt Mar 26, 2019
5d9fb6c
Minor changes
renewiegandt Mar 26, 2019
07d492c
Minor changes
renewiegandt Mar 26, 2019
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Update parameters in README
renewiegandt committed Mar 26, 2019
commit 89ae0576e7ecd3d61f99e36c96f433189aecb1d4
11 changes: 7 additions & 4 deletions README.md
Original file line number Diff line number Diff line change
@@ -54,14 +54,14 @@ Required arguments:
--genome_fasta Path to genome in FASTA-format
--motif_db Path to motif-database in MEME-format
--config Path to UROPA configuration file
--organism Input organism [hg38 | hg19 | mm9 | mm10]
--organism Input organism [hg38 | hg19 | mm9 | mm10]
--out Output Directory (Default: './out/')

Optional arguments:

--help [0|1] 1 to show this help message. (Default: 0)
--gtf_path Path to gtf-file. If path is set the process which creates a gtf-file is skipped.
--tfbs_path Path to directory with output from tfbsscan. If given tfbsscan will be skipped.
--tfbs_path Path to directory with tfbsscan output. If given tfbsscan will be skipped.

Footprint extraction:
--window_length INT This parameter sets the length of a sliding window. (Default: 200)
@@ -78,7 +78,7 @@ Optional arguments:
Sequence preparation/ reduction:
--kmer INT K-mer length (Default: 10)
--aprox_motif_len INT Motif length (Default: 10)
--motif_occurence FLOAT Percentage of motifs over all sequences. Use 1 (Default) to assume every sequence contains a motif.
--motif_occurrence FLOAT Percentage of motifs over all sequences. Use 1 (Default) to assume every sequence contains a motif.
--min_seq_length Interations Remove all sequences below this value. (Default: 10)
Clustering:
--global INT Global (=1) or local (=0) alignment. (Default: 0)
@@ -96,14 +96,17 @@ Optional arguments:
--tomtom_treshold FLOAT Threshold for similarity score. (Default: 0.01)
--best_motif INT Get the best X motifs per cluster. (Default: 3)
--gap_penalty INT Set penalty for gaps in GLAM2 (Default: 1000)
--seed Set seed for GLAM2 (Default: 123456789)
Moitf clustering:
--cluster_motif Boolean If 1 pipeline clusters motifs. If its 0 it does not. (Default: 0)
--cluster_motif Boolean If 1 pipeline clusters motifs. If its 0 it does not. (Defaul: 0)
--edge_weight INT Minimum weight of edges in motif-cluster-graph (Default: 5)
--motif_similarity_thresh FLOAT Threshold for motif similarity score (Default: 0.00001)

Creating GTF:
--tissues List/String List of one or more keywords for tissue-/category-activity, categories must be specified as in JSON
config
Evaluation:
--max_uropa_runs INT Maximum number UROPA runs running parallelized (Default: 10)
All arguments can be set in the configuration files
```