HiC/T2C Sequencing

HiC/T2C-seq

The spatial organisation of chromatin is of high interest in the context of transcription and regulatory units. Topologically associating domains (TAD) are self-interacting genomic regions, wherein DNA is physically interacting.

Challenges

  • HiC: Genome wide genomic interactions (all to all)
  • T2C: Genomic interaction of a target region (many to all)
  • Validating the significans of the data
  • Comparing results from different samples
  • Expensive and difficult procedure in the wet lab

Pipeline


Results