Analysis and visualization of differential methylation in genomic regions

ADMIRE

DNA methylation at cytosine nucleotides constitutes epigenetic gene regulation impacting cellular development and the stage of a disease. Besides whole genome bisulfit sequencing, Illumina HumanMethylationEPIC Assays represent a versatile and cost-effective tool to investigate changes of methylation patterns at CpG sites. ADMIRE is a semi-automatic analysis pipeline and visualization tool for Infinium HumanMethylation450K and Infinium MethylationEpic assays.

Features

Automatic filtering and normalization
Statistical testing and multiple testing correction
Supports arbitrary number of samples and sample groups
Differential methylation analysis on pre-calculated and individual genomic regions
Provides ready-to-plug-in files for genome browsers (like IGV)
Provides publication-ready figures for the most differentially methylated regions
Performs gene set enrichment analysis on predefined and individual gene sets

Interface

The web-based version of ADMIRE provides a simple interface to researchers with limited programming skills, whereas the offline version is suitable for integration into custom pipelines. ADMIRE may be used via our freely available web service at https://bioinformatics.mpi-bn.mpg.de without any limitations concerning the size of a project. An offline version for local execution is available from our website or GitHub.