WobbleDORF

WobbleDORF is an modular structured command line tool to find single nucleotide polymorphisms, that would disrupt an open reading frame. Its designed to work with generic fasta and gtf files and can be used to pinpoint a specific transcript or a whole genome. Main Feature is the preservation of cannonical open reading frames and the usage of wobble bases to disrupt frame different open reading frames inside preserved ones. Furthermore multiple gRNA design tools are integrated, as well as different output formats for visualization.