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proost committed Dec 5, 2017
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19 changes: 13 additions & 6 deletions docs/tutorials/004_compare_specificity.md
Expand Up @@ -4,7 +4,6 @@ Tissue specificity can be combined with gene families to pick up genes with cons
also to detect genes within one species that sub- or neo-functionalized after duplication. Look at the examples below to
see how.


## Conserved Expression

Some low-abudant genes can cause false positives when looking for tissue/condition specificity. These adverse results can
Expand All @@ -16,14 +15,22 @@ Tomato (*Solanum lycopersicum*) fruis. This feature is available from the tools
**Compare specificity**. The SPM (Specificity Metric) slider for tomato is adjusted to yield a similar number of hits
as in *Arabidopsis thaliana*.

![Compare specificity](images/compare_specificity_entry.png "Compare specificity entry")
![Compare specificity entry](images/compare_specificity_entry.png "Compare specificity entry")

In the resulting page orthogroups with a member expressed in one, the other or both selected conditions are shown in a
venn diagram, with a detailed list below. By hovering over a gene, the description can be observed.

![Compare specificity](images/compare_specificity_result.png "Compare specificity result")
![Compare specificity result](images/compare_specificity_result.png "Compare specificity result")

Here, the first reported hit is AGL1 *aka.* SHATTERPROOF 1, a known
[fruit development gene](https://www.ncbi.nlm.nih.gov/pubmed/10903201), clearly illustrating the power of this approach.

## Sub- or Neofunctionalization

Similarly you can compare *Arabidopsis thaliana* root specific genes (default SPM of 0.85) with pollen in the same
species (using the same SPM cutoff).

Here, the first reported hit is AGL1 aka. SHATTERPROOF 1, a known fruit development gene, clearly illustrating the power of this
approach.
![Compare specificity result2](images/compare_specificity_result2.png "Compare specificity result2")

## Sub- or Neofunctionalization
Here **74** orthogroups are reported to show signs of sub- or neo-functionalization. This includes
**Cellulose Synthase (like)** genes included in the example from the [previous section](004_compare_specificity.md).
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